OXIDATION OF LOW-DENSITY-LIPOPROTEIN BY CU-2+ AND LIPOXYGENASE - AN ELECTRON-SPIN-RESONANCE STUDY

被引:48
作者
KALYANARAMAN, B [1 ]
ANTHOLINE, WE [1 ]
PARTHASARATHY, S [1 ]
机构
[1] UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093
关键词
Copper ion; ESR; Free radical; Lipoxygenase; Low density lipoprotein; Oxidation;
D O I
10.1016/0304-4165(90)90090-J
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this work was to obtain spectroscopic evidence for free radicals formed during copper ion- and lipoxygenase-catalyzed oxidation of the low-density lipoprotein. During the initial oxidation phase, a free-radical metabolite derived from the endogeneous α-tocopherol present in the low-density lipoprotein was detected by the electron spin resonance technique. The divalent copper ions were bound to the residual EDTA present in the low-density lipoprotein and to the protein. Production of the α-tocopherol radical was suppressed in the presence of spin traps. Evidence for the low-density lipoprotein-lipid derived radicals was obtained by ESR-spin trapping methods. Implications of these findings in the oxidative modification of the low-density lipoprotein are discussed. © 1990.
引用
收藏
页码:286 / 292
页数:7
相关论文
共 25 条