MICROPELLICULAR STATIONARY PHASES FOR RAPID PROTEIN-ANALYSIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:26
作者
KALGHATGI, K
机构
[1] Department of Chemical Engineering, Yale University, New Haven
来源
JOURNAL OF CHROMATOGRAPHY | 1990年 / 499卷
关键词
D O I
10.1016/S0021-9673(00)96978-4
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The high separating speed, efficiency and opeational stability of various micro-pellicular stationary phases are demonstrated in the high-performance liquid chromatography (HPLC) of biopolymers. The micropellicular sorbents were prepared from 2-μm fluid-impervious silica micropheres as the support, with a thin layer of different retentive materials at the surface. These include a molecular fur of octyl or stearyl chains for reversed-phase chromatography as well as a hydrophilic layer with amino groups and polyethyleneglycol chains for anion-exchange and hydrophobic interaction chromatography, respectively. The use of appropriate micropellicular stationary phases for protein separation by metal-interaction and affinity chromatography is also illustrated. In most cases, operation at elevated column temperature was found to be preferable for rapid separations. Preliminary results show that the stability of micropellicular columns compares very favorably with that of columns conventionally used in HPLC and that they are easy to maintain. © 1990.
引用
收藏
页码:267 / 278
页数:12
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