THE USE OF XENOPUS OOCYTES TO EVALUATE DRUGS AFFECTING BRAIN CA2+ CHANNELS - EFFECTS OF BIFEMELANE AND SEVERAL NOOTROPIC AGENTS

被引：34

作者：

KANEKO, S ^{[1
]}

TAKAHASHI, H ^{[1
]}

SATOH, M ^{[1
]}

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,KYOTO 606,JAPAN

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1990年 / 189卷 / 01期

关键词：

BIFEMELANE; IDEBENONE; VINPOCETINE; CA2+ CHANNELS; OOCYTES (XENOPUS); BRAIN; MESSENGER RNA; (GUINEA-PIG);

D O I：

10.1016/0922-4106(90)90229-Q

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Effects of nootropic or cerebroprotective drugs on voltage-sensitive Ca2+ channels (VSCC) in the mammalian brain were evaluated comparatively by depolarization-evoked Ca2+ channel currents in Xenopus oocytes injected with brain mRNA and by a high K+-stimulated Ca-45 uptake into synaptosomes. It was found that several anti-amnesic agents (bifemelane, idebenone and vinpocetine) inhibited these VSCC-related responses with stronger potency than phenytoin and flurazepam. On the inhibiting potency of various compounds, there was a significant but weak correlation between the results from synaptosomes and those from the injected oocytes, since nifedipine, verapamil and diltiazem at 100-mu-M did not reduce Ca-45 influx in synaptosomes but partly inhibited VSCCs in the oocytes. The blockade of neuronal L- and N-type VSCCs may participate in the anti-ischemic/hypoxic actions of nootropic drugs.

引用

页码：51 / 58

页数：8

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