SYNTHESIS AND PRODUCTION OF POLY(3-HYDROXYVALERIC ACID) HOMOPOLYESTER BY CHROMOBACTERIUM-VIOLACEUM

被引：82

作者：

STEINBUCHEL, A ^{[1
]}

DEBZI, EM ^{[1
]}

MARCHESSAULT, RH ^{[1
]}

TIMM, A ^{[1
]}

机构：

[1] MCGILL UNIV, DEPT CHEM, MONTREAL H3A 2A7, QUEBEC, CANADA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1993年 / 39卷 / 4-5期

关键词：

D O I：

10.1007/BF00205030

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chromobacterium violaceum DSM 30191 accumulated a homopolyester of 3-hydroxyvaleric acid (3HV) up to 65% of the cellular dry matter during cultivation in fed-batch cultures with valeric acid as sole carbon source and during cell starvation of the nitrogen source. From fructose, gluconate, propionate or hexanoate a homopolyester of 3-hydroxybutyrate (3HB) was accumulated. Poly(3HV) homopolyester was also accumulated by two different strains of C. vio violaceum, whereas two other strains of C. violaceum and three strains of Janthinobacterium lividum accumulated poly(3HB-co-3HV) copolyesters from valerate. The composition of the biosynthetic poly(3HV) was confirmed by various nuclear magnetic resonance spectroscopic methods. Differential scanning calorimetry analysis of four poly(3HV) samples that were isolated from different batches of cells revealed glass transition temperatures between -10 and -12-degrees-C and melting points between 107 and 112-degrees-C. Viscosity measurements gave intrinsic viscosities between 62.5 and 124.8 x 10(-2) dl/g for these samples, indicating approximate relative molecular masses between 60000 and 145000 of the biosynthetic poly(3HV).

引用

页码：443 / 449

页数：7

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