ANALYSIS OF THE TOPOLOGY OF A MEMBRANE-PROTEIN BY USING A MINIMUM NUMBER OF ALKALINE-PHOSPHATASE FUSIONS

被引：80

作者：

BOYD, D

TRAXLER, B

BECKWITH, J

机构：

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 02期

关键词：

D O I：

10.1128/JB.175.2.553-556.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An approach to analyzing the topology of membrane proteins with alkaline phosphatase fusions is described. Precise fusions were constructed by using polymerase chain reaction at the C terminus of each hydrophilic region of the membrane protein. The disruption of topogenic signals is thereby minimized, and predictable anomalous results are avoided. The Escherichia coli MalG protein has been analyzed.

引用

页码：553 / 556

页数：4

共 23 条

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