DETECTION OF CYTOCHROME-P450-1A1 IN NORTH-SEA DAB LIVER AND KIDNEY

被引：9

作者：

GOKSOYR, A ^{[1
]}

LARSEN, HE ^{[1
]}

BLOM, S ^{[1
]}

FORLIN, L ^{[1
]}

机构：

[1] UNIV GOTEBORG, DEPT ZOOPHYSIOL, S-41390 GOTHENBURG, SWEDEN

来源：

MARINE ECOLOGY PROGRESS SERIES | 1992年 / 91卷 / 1-3期

关键词：

D O I：

10.3354/meps091083

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Liver and kidney tissue of dab Limanda limanda were analyzed during the Bremerhaven Workshop by immunochemical and catalytic assays for cytochrome P450 1A1 induction. Antibodies to cod P450 1A1 cross-reacted with a protein of M(r) = 58 000 daltons in the dab liver samples, suggested to be the dab P450 1A1 protein. By employing a recently developed indirect ELISA with these antibodies, we observed elevated levels of P450 1A1 protein in the innermost stations of the German Bight transect in both liver and kidney (head and trunk kidney) samples. Ethoxyresorufin O-deethylase (EROD) activities in trunk kidney were also elevated in the innermost stations. Along the drilling station gradient no induction could be detected. Immuno-detection of the cytochrome P450 1A1 induction response should provide a convenient indicator for biological effects of several classes of organic contaminants.

引用

页码：83 / 88

页数：6

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