HYDRODYNAMIC AND SIZE-EXCLUSION CHROMATOGRAPHY OF POLYMERS ON POROUS PARTICLES

When packed columns filled with porous particles are used for the separation of macromolecules, either size-exclusion chromatography (SEC), hydrodynamic chromatography (HDC) or a combination of the two determine the migration rate. A simple theoretical model, assuming a stagnant pore liquid, was developed to describe a molecular weight calibration graph, which includes both HDC and SEC. In the overall calibration graph, there is a transition from the HDC calibration region at higher molecular weights to an SEC region at lower molecular weights. The smoothness of the transition depends on the ratio of the particle diameter to the pore diameter.