SIMULTANEOUS DETERMINATION OF PHOSPHATE AND PYROPHOSPHATE BY AN AMPEROMETRIC FLOW-INJECTION SYSTEM WITH IMMOBILIZED ENZYME REACTORS

被引：8

作者：

YAO, T

WASA, T

机构：

[1] Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture, Osaka, 593, Gakuen‐cho, Sakai

来源：

ELECTROANALYSIS | 1993年 / 5卷 / 9-10期

关键词：

ENZYME REACTOR; PHOSPHATE; FLOW INJECTION ANALYSIS;

D O I：

10.1002/elan.1140050926

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A bioamperometric flow-injection system is proposed for the simultaneous determination of phosphate and pyrophosphate. The injected sample is split so that part passes through an immobilized inorganic pyrophosphatase reactor before passing through a coimmobilized nucleoside phosphorylase-xanthine oxidase reactor. The other portion passes only through the latter reactor. Because each channel has a different residence time, two peaks are obtained. The first peak corresponds to phosphate and the second peak to the total phosphate (phosphate plus pyrophosphate). The maximum currents of both peaks are linearly related to the concentration of phosphate and total phosphate, respectively; 20 samples per hour can be processed with an RSD < 1.5%.

引用

页码：887 / 890

页数：4

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