PURIFIED GLUTATHIONE S-TRANSFERASES FROM PARASITES AS CANDIDATE PROTECTIVE ANTIGENS

被引：44

作者：

SHARP, PJ ^{[1
]}

SMITH, DRJ ^{[1
]}

BACH, W ^{[1
]}

WAGLAND, BM ^{[1
]}

COBON, GS ^{[1
]}

机构：

[1] CSIRO,MCMASTER LAB,DIV ANIM HLTH,GLEBE,NSW 2037,AUSTRALIA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1991年 / 21卷 / 07期

关键词：

LUCILIA-CUPRINA; HAEMONCHUS-CONTORTUS; GLUTATHIONE S-TRANSFERASE; INHIBITOR; ANTISERA; AMINO ACID ANALYSIS; AMINO ACID SEQUENCE;

D O I：

10.1016/0020-7519(91)90152-W

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Glutathione S-transferase (GST) activity was detected in larvae of the Australian sheep blowfly Lucilia cuprina, and in the nematode Haemonchus contortus. A specific inhibitor of the enzyme was shown to affect survival of both species of parasite in vitro. GST from both parasites has been purified and partially characterized. Antisera raised to the purified enzymes were shown to inhibit the enzyme activity in vitro. However, the antisera had no effect on the survival of either parasite.

引用

页码：839 / 846

页数：8

共 22 条

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