MULTIPLE XENOBIOTIC-INDUCIBLE P450S ARE INVOLVED IN ALKOXYCOUMARIN AND ALKOXYRESORUFIN METABOLISM IN HIGHER-PLANTS

被引：14

作者：

BATARD, Y ^{[1
]}

ZIMMERLIN, A ^{[1
]}

LERET, M ^{[1
]}

DURST, F ^{[1
]}

WERCKREICHHART, D ^{[1
]}

机构：

[1] INST BIOL MOLEC PLANTES, DEPT ENZYMOL CELLULAIRE & MOLEC, CNRS, UPR 406, F-67000 STRASBOURG, FRANCE

来源：

PLANT CELL AND ENVIRONMENT | 1995年 / 18卷 / 05期

关键词：

HELIANTHUS TUBEROSUS; ALKOXYCOUMARINS; ALKOXYPHENOXAZONES; ALKOXYRESORUFINS; ANTIBODIES; COMPOSITAE; CYTOCHROME P450; DEALKYLATION; INDUCTION; PURIFICATION; XENOBIOTICS;

D O I：

10.1111/j.1365-3040.1995.tb00552.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

O-Dealkylation of two series of fluorescent 7-alkoxycoumarins and 7-alkoxyphenoxazones by plant cytochrome P450s was investigated in Helianthus tuberosus tuber tissues treated with prototype P450 inducers, environmental pollutants or agrochemicals, Methoxy-, ethoxy-, propoxy- and butoxycoumarins and methoxy- and ethoxyresorufins were metabolized by fplant microsomes, Dealkylation of pentoxy- and benzyloxyresorufins was not detected, All dealkylating activities were enhanced by aging plant tissues in the presence of xenobiotics, in some cases up to 20-fold relative to the activities detected in control tissues, Increases in total P450 in the same tissues never exceeded 3-fold. The isozymes induced by prototype P450 inducers clearly differed from those in mammalian liver, That multiple P450s with overlapping substrate specificities were involved in the metabolism of both alkoxycoumarins and alkoxyresorufins was demonstrated by (1) the differential induction of the activities in response to exposure to xenobiotics, (2) the differential inhibition of the activities by clotrimazole, paclobutrazole and tetcyclacis in aminopyrine and benzo(a)pyrene-treated tissues, and (3) the selective inhibition observed with antibodies raised against purified ethoxycoumarin deethylase fractions, Our results suggest that the measurement of the dealkylation of such fluorescent substrates in plants might be useful to monitor environmental pollution.

引用

页码：523 / 533

页数：11

共 38 条

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