DIAMIDE, A NEW REAGENT FOR INTRACELLULAR OXIDATION OF GLUTATHIONE TO DISULFIDE

被引：525

作者：

KOSOWER, NS

KOSOWER, EM

WERTHEIM, B

CORREA, WS

机构：

[1] Departments of Biological Ultrastructure and Biophysics, The Weizmann Institute of Science, Rehovot

[2] Department of Chemistry, State University of New York at Stony Brook, Stony Brook

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 37卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(69)90850-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new thiol-oxidizing agent, diamide, (CH3)2NCONNCON(CH3)2, is shown to stoichiometrically oxidize glutathione (GSH) within the human red blood cell to the disulfide (GSSG). Conversion of GSH to GSSG is very rapid, even at 1-40. No inter ference with cellular function is observed as shown by almost complete regeneration of GSH after incubation with glucose at 370, and no alteration in hemoglobin, osmotic fragility or cell density is found. © 1969.

引用

页码：593 / &

共 13 条

[1]

BEUTLER E, 1957, J LAB CLIN MED, V49, P84

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