CLONING AND EXPRESSION OF THE DELAYED-RECTIFIER ISK CHANNEL FROM NEONATAL RAT-HEART AND DIETHYLSTILBESTROL-PRIMED RAT UTERUS

被引：190

作者：

FOLANDER, K

SMITH, JS

ANTANAVAGE, J

BENNETT, C

STEIN, RB

SWANSON, R

机构：

[1] MERCK SHARP & DOHME LTD,DEPT PHARMACOL,W26-200B,W POINT,PA 19486

[2] MERCK SHARP & DOHME LTD,DEPT BIOL CHEM,W POINT,PA 19486

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 08期

关键词：

Antisense inhibition; Polymerase chain reaction; Voltage clamp; Xenopus oocytes;

D O I：

10.1073/pnas.87.8.2975

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

cDNAs encoding a delayed-rectifier-type K+ channel were cloned from both neonatal rat heart and ovariectomized, diethylstilbestrol-primed rat uterus by using the polymerase chain reaction. Both clones have nucleotide sequences identical to that encoding the rat kidney IsK channel [Takumi, T., Ohkubo, H. & Nakanishi, S. (1988) Science 242, 1042-1045] and encode a putative protein of 130 amino acids. Injection of RNA transcripts of the cDN As into Xenopus oocytes resulted in the expression of a slowly activating, voltage-dependent K+ current. An antisense oligonucleotide, derived from the sequence of the clone, specifically inhibited the expression of the slow, outward current observed in cells injected with mRNAs isolated from the parent tissues (i.e., kidney, heart, and uterus), indicating that the cloned gene underlies the major K+ current expressed from RNA isolated from these tissues.

引用

页码：2975 / 2979

页数：5

共 36 条

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