A FACTOR THAT SPECIFICALLY BINDS TO THE 5'-UNTRANSLATED REGION OF ENCEPHALOMYOCARDITIS VIRUS-RNA

被引：49

作者：

BOROVJAGIN, AV

EVSTAFIEVA, AG

UGAROVA, TY

SHATSKY, IN

机构：

[1] MV LOMONOSOV STATE UNIV, AN BELOZERSKY LAB, DIV VIRUS CELL INTERACT, LENINSKY HILLS, MOSCOW 119899, USSR

[2] MV LOMONOSOV STATE UNIV, AN BELOZERSKY LAB, DIV CHEM & BIOCHEM NUCLEOPROT, MOSCOW 119899, USSR

来源：

FEBS LETTERS | 1990年 / 261卷 / 02期

关键词：

Encephalomyocarditis virus; Internal initiation; Protein-RNA interaction; Translation;

D O I：

10.1016/0014-5793(90)80561-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein factor that specifically binds to the 5'-untranslated region of encephalomyocarditis virus (EMCV) RNA has been found in extracts of ascites carcinoma Krebs-2 cells. This was done using UV-irradiation on extracts supplemented with in vitro synthesized 32P-labelled transcripts followed by analysis of crosslinked proteins by SDS-polyacrylamide gel electrophoresis. The transcripts represented the viral RNA sequence from nt 315 to 1155, its derivatives with internal deletions or truncated forms. This set of transcripts has allowed us to find out that the factor (p58) binds to EMCV RNA within the sequence 315-485. © 1990.

引用

页码：237 / 240

页数：4

共 22 条

[1] 5'-TERMINAL CAP STRUCTURE IN EUKARYOTIC MESSENGER RIBONUCLEIC-ACIDS [J].

BANERJEE, AK .

MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :175-205

[2] CLONING AND EXPRESSION OF THE GENE FOR BACTERIOPHAGE-T7 RNA-POLYMERASE [J].

DAVANLOO, P ;

ROSENBERG, AH ;

DUNN, JJ ;

STUDIER, FW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :2035-2039

[3]

EVSTAFIEVA AG, 1990, UNPUB NUCLEIC ACIDS

[4] GENOME-LINKED PROTEIN OF PICORNAVIRUSES .4. DIFFERENCE IN VPGS OF ENCEPHALOMYOCARDITIS VIRUS AND POLIOVIRUS AS EVIDENCE THAT GENOME-LINKED PROTEINS ARE VIRUS-CODED [J].

GOLINI, F ;

NOMOTO, A ;

WIMMER, E .

VIROLOGY, 1978, 89 (01) :112-118

[5] ULTRAVIOLET LIGHT-INDUCED CROSSLINKING OF MESSENGER-RNA TO PROTEINS [J].

GREENBERG, JR .

NUCLEIC ACIDS RESEARCH, 1979, 6 (02) :715-732

[6] A SEGMENT OF THE 5' NONTRANSLATED REGION OF ENCEPHALOMYOCARDITIS VIRUS-RNA DIRECTS INTERNAL ENTRY OF RIBOSOMES DURING INVITRO TRANSLATION [J].

JANG, SK ;

KRAUSSLICH, HG ;

NICKLIN, MJH ;

DUKE, GM ;

PALMENBERG, AC ;

WIMMER, E .

JOURNAL OF VIROLOGY, 1988, 62 (08) :2636-2643

[7]

KARASEV AV, 1989, MOL BIOL+, V23, P88

[8] ELECTROPHORETIC SEPARATION OF COMPLEXES INVOLVED IN THE SPLICING OF PRECURSORS TO MESSENGER-RNAS [J].

KONARSKA, MM ;

SHARP, PA .

CELL, 1986, 46 (06) :845-855

[9] HOW DO EUKARYOTIC RIBOSOMES SELECT INITIATION REGIONS IN MESSENGER-RNA [J].

KOZAK, M .

CELL, 1978, 15 (04) :1109-1123

[10] POSSIBLE ROLE OF FLANKING NUCLEOTIDES IN RECOGNITION OF THE AUG INITIATOR CODON BY EUKARYOTIC RIBOSOMES [J].

KOZAK, M .

NUCLEIC ACIDS RESEARCH, 1981, 9 (20) :5233-5252

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