MOUSE-HUMAN CHIMERIC MONOCLONAL-ANTIBODY TO CARCINOEMBRYONIC ANTIGEN (CEA) - INVITRO AND INVIVO ACTIVITIES

被引:24
作者
KOGA, H
KANDA, H
NAKASHIMA, M
WATANABE, Y
ENDO, K
WATANABE, T
机构
[1] KYUSHU UNIV,INST MED BIOREGULAT,3-1-1 MAIDASHI,HIGASHI KU,FUKUOKA 812,JAPAN
[2] KYOTO UNIV,FAC MED,DEPT NUCL ENGN,KYOTO 606,JAPAN
来源
HYBRIDOMA | 1990年 / 9卷 / 01期
关键词
D O I
10.1089/hyb.1990.9.43
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Mouse-human chimeric antibody specific for human carcinoembryonic antigen (CEA) was produced by recombinant DNA techniques. The genes of the mouse variable regions of heavy and light chains were cloned from the mouse hybridoma, 2.7.1G.10., which secreted anti-human CEA antibody (IgG1, κ), and were joined with human γ1 and κ constant genes. The affinity of the resultant chimeric antibody to its relevant antigen was the same as that of the parental mouse monoclonal antibody when analysed by Scatchard plot analysis. The chimeric antibody showed a potent antibody dependent cell-mediated cytotoxicity (ADCC) activity with human peripheral blood mononuclear cells against CEA-positive human adenocarcinoma cells. In vivo imaging analysis revealed that the present chimeric antibody was specifically localized on the tumor site. These results indicate that our mouse-human chimeric antibody is a promising reagent for the diagnosis and therapy of CEA-positive human cancers.
引用
收藏
页码:43 / 56
页数:14
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