RETENTION BEHAVIOR OF BENZENE-DERIVATIVES ON BONDED REVERSED-PHASE COLUMNS

The retention behaviour of fourteen benzene derivatives has been studied in reversed-phase chromatography, eluting them with various aqueous organic solvent systems from the hydrocarbon chemically bonded reversed phase using a micro-chromatograph. The effects of eluent composition and of packings are discussed using the capacity factor, k. A linear relationship ws observed between log k and the logarithm of the molarity of the organic moiety, log [C], in the mobile phase. A p value for each solute was obtained from the slope of the linear relationship of log k vs. log [C]. This value was recognized as the number of the released solvent molecules after the formation of the bristle-solvent-solute complex when the solvated solute molecules were adsorbed on the solvated bristles of the bonded materials. Consideration of p offered a key to the explanation of the role of the eluent on chemically bonded reversed-phase chromatography. © 1979.