AMPEROMETRIC ENZYME ELECTRODE FOR THEOPHYLLINE

被引：37

作者：

WANG, J ^{[1
]}

DEMPSEY, E ^{[1
]}

OZSOZ, M ^{[1
]}

SMYTH, MR ^{[1
]}

机构：

[1] DUBLIN CITY UNIV,SCH CHEM SCI,DUBLIN 9,IRELAND

来源：

ANALYST | 1991年 / 116卷 / 10期

关键词：

THEOPHYLLINE; BIOSENSOR; THEOPHYLLINE OXIDASE ENZYME ELECTRODE; AMPEROMETRY;

D O I：

10.1039/an9911600997

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric biosensor for theophylline, based on the recently isolated enzyme theophylline oxidase, is described. The enzyme is entrapped, together with a ferricytochrome C cofactor, within a polymeric (Nafion) coating. The anodic detection (at + 0.4 V versus Ag-AgCl) is facilitated by the addition of a redox-mediating hexacyanoferrate(III) ion. The influence of various experimental variables is described. The limit of detection is 2 x 10(-6) mol dm-3 theophylline, with linearity prevailing up to 3 x 10(-4) mol dm-3. The fast response and wash times permit rapid flow-injection measurements, with a frequency of 180 samples h-1 and a relative standard deviation of 3.0-4.0%. Prospects of using this electrode for clinical diagnostics are discussed.

引用

页码：997 / 999

页数：3

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