ISOLATION OF PIGMENT-FREE BULK LIPIDS FROM THYLAKOIDS

被引：9

作者：

BRATT, CE ^{[1
]}

AKERLUND, HE ^{[1
]}

机构：

[1] UNIV LUND,POB 7007,S-22007 LUND 7,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1165卷 / 03期

关键词：

PLANT LIPID; PURIFICATION; THYLAKOID; SILICIC ACID CHROMATOGRAPHY; (SPINACH);

D O I：

10.1016/0005-2760(93)90138-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipids from spinach thylakoids were extracted with chloroform/methanol and separated from pigments in a single chromatographic step run at 5-degrees-C using silicic acid adjusted to pH 8. The isolated lipid fraction contained essentially the same amounts of individual lipids as in the initial extract. It contained less than 0.1% of the initial chlorophylls and carotenoids.

引用

页码：288 / 290

页数：3

共 10 条

[1] QUANTITATIVE SEPARATION OF SPINACH THYLAKOIDS INTO PHOTOSYSTEM-II-ENRICHED INSIDE-OUT VESICLES AND PHOTOSYSTEM-I-ENRICHED RIGHT-SIDE-OUT VESICLES [J].