CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST PUTATIVE COLONIZATION FACTORS OF ENTEROTOXIGENIC ESCHERICHIA-COLI AND THEIR USE IN AN EPIDEMIOLOGIC-STUDY

被引:76
作者
VIBOUD, GI
BINSZTEIN, N
SVENNERHOLM, AM
机构
[1] GOTHENBURG UNIV, DEPT MED MICROBIOL & IMMUNOL, S-41346 GOTHENBURG, SWEDEN
[2] INST NACL MICROBIOL CARLOS G MALBRAN, RA-1281 BUENOS AIRES, DF, ARGENTINA
关键词
D O I
10.1128/JCM.31.3.558-564.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Monoclonal antibodies (MAbs) against five putative colonization factors (PCFs), i.e., colonization factor antigen (CFA)/III, coli surface antigen (CS)7 and CS17, PCFO159, and PCFO166 of enterotoxigenic Escherichia coli (ETEC), were produced. Hybridomas (one each) producing specific antibodies against the respective PCFs were selected. All the MAbs reacted with the corresponding fimbriae but not with CFA/I, CFA/II, or CFA/IV or the heterologous PCFs in bacterial agglutination and enzyme-linked immunosorbent assays (ELISAs). In immunoelectron microscopy these MAbs bound along the fimbriae, and they also reacted with the corresponding subunits in immunoblots. The five MAbs were used to evaluate the prevalence of CFA/III, CS7, CS17, PCFO159, and PCFO166 in ETEC strains isolated from children with diarrhea in Argentina. One hundred five ETEC isolates negative for CFA/I, CFA/II, and CFA/IV were tested in slide agglutination or in a dot blot test for spontaneously agglutinating strains; positive results were confirmed by inhibition ELISAs. It was found that 27% of the CFA-negative ETEC strains carried one of the PCFs. The sensitivity of slide agglutination with these MAbs was similar to that with specific polyclonal antisera; however, the specificity was higher. PCFO166 was found in 9.5% of the strains tested, mainly in ETEC of serogroup 078 producing heat-stable toxin alone. CS17 and CS7 were identified in 6.7 and 5.7%, respectively, of strains producing heat-labile toxin only, most of which belonged to serogroup 0114. PCFO159 was found in 3.8% of the isolates tested, whereas CFA/III was detected in only one ETEC strain.
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页码:558 / 564
页数:7
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共 36 条
  • [1] AGUERO ME, 1985, J CLIN MICROBIOL, V22, P576
  • [2] NEW ADHESIVE FACTOR (ANTIGEN-8786) ON A HUMAN ENTEROTOXIGENIC ESCHERICHIA-COLI O117-H4 STRAIN ISOLATED IN AFRICA
    AUBEL, D
    DARFEUILLE-MICHAUD, A
    JOLY, B
    [J]. INFECTION AND IMMUNITY, 1991, 59 (04) : 1290 - 1299
  • [3] COLONIZATION FACTORS OF ENTEROTOXIGENIC ESCHERICHIA-COLI ISOLATED FROM CHILDREN WITH DIARRHEA IN ARGENTINA
    BINSZTEIN, N
    JOUVE, MJ
    VIBOUD, GI
    MORAL, LL
    RIVAS, M
    ORSKOV, I
    AHREN, C
    SVENNERHOLM, AM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (09) : 1893 - 1898
  • [4] BLACK RE, 1986, DEV VACCINES DRUGS D, P23
  • [5] COLONIZATION FACTORS ASSOCIATED WITH ENTERO-TOXIGENIC ESCHERICHIA-COLI ISOLATED IN THAILAND
    CHANGCHAWALIT, S
    ECHEVERRIA, P
    TAYLOR, DN
    LEKSOMBOON, U
    TIRAPAT, C
    EAMPOKALAP, B
    ROWE, B
    [J]. INFECTION AND IMMUNITY, 1984, 45 (02) : 525 - 527
  • [6] IDENTIFICATION OF A NONFIMBRIAL ADHESIVE FACTOR OF AN ENTEROTOXIGENIC ESCHERICHIA-COLI STRAIN
    DARFEUILLE-MICHAUD, A
    FORESTIER, C
    JOLY, B
    CLUZEL, R
    [J]. INFECTION AND IMMUNITY, 1986, 52 (02) : 468 - 475
  • [7] SEROTYPES OF ATTACHMENT PILI OF ENTERO-TOXIGENIC ESCHERICHIA-COLI ISOLATED FROM HUMANS
    DENEKE, CF
    THORNE, GM
    GORBACH, SL
    [J]. INFECTION AND IMMUNITY, 1981, 32 (03) : 1254 - 1260
  • [8] PRODUCTION OF MONOCLONAL-ANTIBODIES - STRATEGY AND TACTICS
    DESTGROTH, SF
    SCHEIDEGGER, D
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1980, 35 (1-2) : 1 - 21
  • [9] PURIFICATION AND CHARACTERIZATION OF THE CFA-I ANTIGEN OF ENTEROTOXIGENIC ESCHERICHIA-COLI
    EVANS, DG
    EVANS, DJ
    CLEGG, S
    PAULEY, JA
    [J]. INFECTION AND IMMUNITY, 1979, 25 (02) : 738 - 748
  • [10] PLASMID-CONTROLLED COLONIZATION FACTOR ASSOCIATED WITH VIRULENCE IN ESCHERICHIA-COLI ENTEROTOXIGENIC FOR HUMANS
    EVANS, DG
    SILVER, RP
    EVANS, DJ
    CHASE, DG
    GORBACH, SL
    [J]. INFECTION AND IMMUNITY, 1975, 12 (03) : 656 - 667