CONSTRUCTION OF AN INFECTIOUS DNA CLONE OF THE Y-1 STRAIN OF CANINE PARVOVIRUS AND CHARACTERIZATION OF THE VIRUS DERIVED FROM THE CLONE

被引：9

作者：

HORIUCHI, M

SHINAGAWA, M

机构：

[1] Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido

来源：

ARCHIVES OF VIROLOGY | 1993年 / 130卷 / 3-4期

关键词：

D O I：

10.1007/BF01309656

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have cloned genome fragments of canine parvovirus strain Y 1 from replicative-form DNA and double-stranded DNA synthesized from virion DNA in vitro, and constructed a recombinant plasmid containing a full-length Y 1 genome (pCPVY 1). When this recombinant plasmid was transfected into cell cultures, an infectious virus could be recovered. To characterize this pCPVY 1-derived virus, its biological properties were compared with those of the parental strain. No difference was observed between them in antigen expression, viral DNA replication, hemagglutination ability, and virus multiplication, indicating that the virus derived from the infectious plasmid inherited the biological properties of the authentic Y 1 strain. Therefore, this recombinant plasmid appears to be useful for reverse genetics of canine parvovirus.

引用

页码：227 / 236

页数：10

共 33 条

[1] CHARACTERIZATION OF THE CELL TYPE-SPECIFIC DETERMINANT IN THE GENOME OF MINUTE VIRUS OF MICE [J].

ANTONIETTI, JP ;

SAHLI, R ;

BEARD, P ;

HIRT, B .

JOURNAL OF VIROLOGY, 1988, 62 (02) :552-557

[2] NUCLEOTIDE-SEQUENCE OF THE 5'-TERMINAL PALINDROME OF ALEUTIAN MINK DISEASE PARVOVIRUS AND CONSTRUCTION OF AN INFECTIOUS MOLECULAR CLONE [J].

BLOOM, ME ;

ALEXANDERSEN, S ;

GARON, CF ;

MORI, S ;

WEI, W ;

PERRYMAN, S ;

WOLFINBARGER, JB .

JOURNAL OF VIROLOGY, 1990, 64 (07) :3551-3556

[3] CLONING AND SEQUENCE OF DNA ENCODING STRUCTURAL PROTEINS OF THE AUTONOMOUS PARVOVIRUS FELINE PANLEUKOPENIA VIRUS [J].

CARLSON, J ;

RUSHLOW, K ;

MAXWELL, I ;

MAXWELL, F ;

WINSTON, S ;

HAHN, W .

JOURNAL OF VIROLOGY, 1985, 55 (03) :574-582

[4]

CARMICHAEL LE, 1980, AM J VET RES, V41, P784

[5] MUTATION OF A CONSENSUS PURINE NUCLEOTIDE BINDING-SITE IN THE ADENOASSOCIATED VIRUS REP GENE GENERATES A DOMINANT NEGATIVE PHENOTYPE FOR DNA-REPLICATION [J].

CHEJANOVSKY, N ;

CARTER, BJ .

JOURNAL OF VIROLOGY, 1990, 64 (04) :1764-1770

[6]

CRANDELL RA, 1973, IN VITRO CELL DEV B, V9, P176, DOI 10.1007/BF02618435

[7] MAPPING OF THE FIBROTROPIC AND LYMPHOTROPIC HOST RANGE DETERMINANTS OF THE PARVOVIRUS MINUTE VIRUS OF MICE [J].

GARDINER, EM ;

TATTERSALL, P .

JOURNAL OF VIROLOGY, 1988, 62 (08) :2605-2613

[8]

GOTO H, 1984, JPN J VET SCI, V46, P519

[9]

HIGASHIHARA T, 1981, JPN J VET SCI, V43, P841

[10] CHARACTERIZATION OF THE STAGE(S) IN THE VIRUS-REPLICATION CYCLE AT WHICH THE HOST-CELL SPECIFICITY OF THE FELINE PARVOVIRUS SUBGROUP IS REGULATED IN CANINE CELLS [J].

HORIUCHI, M ;

ISHIGURO, N ;

GOTO, H ;

SHINAGAWA, M .

VIROLOGY, 1992, 189 (02) :600-608

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