SEPARATION AND IMPROVEMENT IN DETECTION OF POLYCYCLIC AROMATIC-HYDROCARBONS BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING MICELLAR MOBILE-PHASE AND FLUORESCENCE DETECTOR

被引：22

作者：

HADJMOHAMMADI, MR

FATEMI, MH

机构：

[1] Institute of Chemistry, Mazandaran University, Babolsar

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 13期

关键词：

D O I：

10.1080/10826079508009309

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Separation and investigation of Detection Limit (LOD)of Polycyclic Aromatic Hydrocarbons,PAH,compounds with RP-HPLC using fluorescence detection were studied Lower LOD for these compounds were observed because of higher fluorescence intensity in the prescence of micellar mobile phase as compared to those of hydroorganics. The codition for optimum separation and enhancement of fluorescence intensity by variation of concentration of coorganic solvent (2-Propanol) and SDS were obtained. Selectivity factor and fluorescence intensity were the basic factors for the optimization of separation and improvement in detection respectively. The condition obtined were 3% of 2-Propanol in 0.035 M SDS.

引用

页码：2569 / 2578

页数：10

共 12 条

[1] USE OF AN AQUEOUS MICELLAR MOBILE PHASE FOR SEPARATION OF PHENOLS AND POLYNUCLEAR AROMATIC-HYDROCARBONS VIA HPLC [J].