INFLUENCE OF HUMAN GROWTH HORMONE ON GLYCOLYSIS OF HUMAN RED CELLS IN VITRO

The influence of human growth hormone on glucose consumption, and some enzymes and metabolites of glycolysis of human red cells were studied in vitro. It has been shown that human growth hormone caused: 1. 1. about 54% inhibition of the glucose consumption; 2. 2. correspondingly, diminished lactic acid production; 3. 3. accumulation of glucose-6-phosphate and fructose-6-phosphate due to inhibition of phosphofructokinase; 4. 4. using the glycolysis-activating effect of phosphate buffer, adenosine triphosphate production was depressed, but using the Krebs-Ringer-bicarbonate buffer, the diminution was lower than in the controls, without addition of the growth hormone; 5. 5. increased pyruvate production with less decreased pyruvate kinase and less increased lactic dehydrogenase activity. Possible connections in the course of the glycolytic process in human red cells are discussed. © 1969.