COMPARISON OF SELECTIVITIES OF REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, CAPILLARY ZONE ELECTROPHORESIS AND MICELLAR CAPILLARY ELECTROPHORESIS IN THE SEPARATION OF NEUROHYPOPHYSEAL PEPTIDES AND ANALOGS

被引：35

作者：

SUTCLIFFE, N ^{[1
]}

CORRAN, PH ^{[1
]}

机构：

[1] NATL INST BIOL STAND & CONTROLS,DIV CHEM,BLANCHE LANE,POTTERS BAR EN6 3QG,HERTS,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 636卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)80061-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A number of RP-HPLC systems, including those prescribed in several official monographs, have been evaluated for separating oxytocin, the vasopressins, some clinically important analogues and two additional neurohypophyseal nonapeptides. The separation has been compared with capillary zone electrophoresis and micellar electrophoresis in four micellar systems: cationic, anionic, zwitter-ionic and neutral. Complete separation was achieved by both RP-HPLC and micellar CE but the importance of charge as a major parameter of separation in CE confers a distinct and useful selectivity to micellar CE based separations.

引用

页码：95 / 103

页数：9

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