ISOLATION OF THEILERIA-SERGENTI PIROPLASMS FROM INFECTED ERYTHROCYTES AND DEVELOPMENT OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR SERODIAGNOSIS OF THEILERIA-SERGENTI INFECTIONS

被引：43

作者：

SHIMIZU, S ^{[1
]}

SUZUKI, K ^{[1
]}

NAKAMURA, K ^{[1
]}

KADOTA, K ^{[1
]}

FUJISAKI, K ^{[1
]}

ITO, S ^{[1
]}

MINAMI, T ^{[1
]}

机构：

[1] NATL INST ANIM HLTH, TUKUBA, IBARAKI 305, JAPAN

来源：

RESEARCH IN VETERINARY SCIENCE | 1988年 / 45卷 / 02期

关键词：

D O I：

10.1016/S0034-5288(18)30932-9

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A method of obtaining a pure suspension of Theileria sergenti piroplasms from infected bovine erythrocytes was developed and the resulting parasites used as antigen in an enzyme-linked immunosorbent assay (ELISA). Piroplasms were freed from infected erythrocytes using the nitrogen cavitation technique and then separated from unbroken erythrocytes by centrifugation at 670 gmax. The parasites in the supernatant were then sedimented by centrifugation at 2700 gmax and the purified fraction examined by electron microscopy. This examination showed that the isolated piroplasms were morphologically intact and that there were morphologically intact and that there are few contaminants. SDS-PAGE and spectrophotometric analysis showed that this fraction contained little erythrocyte component. The piroplasms thus obtained were sonicated and treated with Triton X-100 then used for ELISA antigen. The ELISA values had a high correlation with titres obtained using the indirect fluorescent antibody test on sera from cattle infected with T. sergenti.

引用

页码：206 / 212

页数：7

共 17 条

[1] PURIFICATION AND CHARACTERIZATION OF PRECIPITATING ANTIGENS FROM THEILERIA-PARVA [J].