INDUCTION OF RAT-LIVER MICROSOMAL-ENZYMES BY CYCLOHEXIMIDE

Administration of cycloheximide into rats induced certain enzymes of rough and smooth microsomes. NADPH cytochrome c reductase of smooth microsomes and glucose-6-phosphatase of rough and smooth microsomes attained peak activities 2 hr after cycloheximide treatment. Cytochrome P-450 and cytochrome b5 of the rough microsomes were highest 6 hr after the antibiotic injection. On the other hand, cycloheximide reduced ATPase activity in the smooth microsomes. All the components studied except NADPH cytochrome c reductase of the smooth microsomes approached control values 24 hr after the treatment. The responses of rough and smooth microsomes toward cycloheximide were different. © 1979.