EVALUATION OF A RAPID, SENSITIVE AND SPECIFIC ASSAY FOR THE DETERMINATION OF COLLAGENOLYTIC ACTIVITY IN BIOLOGICAL SAMPLES

被引：18

作者：

LEFEVERE, MF

SLEGERS, GA

CLAEYS, AE

机构：

[1] Laboratorium voor Analytische Chemie, Faculteit Farmaceutische Wetenschappen, Rijksuniversiteit Gent, B-9000 Gent, De Pintelaan

来源：

CLINICA CHIMICA ACTA | 1979年 / 92卷 / 02期

关键词：

D O I：

10.1016/0009-8981(79)90111-6

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 [基础医学];

摘要：

Several methods for the determination of collagenolytic activity were compared from the point of view of sensitivity, selectivity, simplicity and practical value for large numbers of biological samples. A labelled collagen substrate was prepared using [3H] acetic anhydride. The specificity of the assay as well as conditions allowing an optimum detection limit were investigated. The influence of low temperatures, lyophilisation and salt concentration on Clostridium histolyticum collagenase have been investigated. © 1979.

引用

页码：167 / 175

页数：9

共 9 条

[1]

BERMAN MB, 1973, ANAL BIOCHEM, V54, P522, DOI 10.1016/0003-2697(73)90384-9

[2]

RAPID SENSITIVE COLLAGENASE ASSAY [J].

GISSLOW, MT ;

MCBRIDE, BC .

ANALYTICAL BIOCHEMISTRY, 1975, 68 (01) :70-78

[3]

KAUFMAN EJ, 1965, P SOC EXP BIOL MED, V120, P632

[4]

SENSITIVE ASSAY FOR COLLAGENOLYTIC ACTIVITY USING TRITIATED COLLAGEN [J].

LABROSSE, KR ;

LIENER, IE ;

HARGRAVE, PA .

ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) :218-223

[5]

TADPOLE COLLAGENASE . PREPARATION AND PURIFICATION [J].

NAGAI, Y ;

LAPIERE, CM ;

GROSS, J .

BIOCHEMISTRY, 1966, 5 (10) :3123-+

[6]

REPRODUCIBLE QUANTITATIVE COLLAGENASE RADIOFIBRIL ASSAY [J].

ROBERTSON, PB ;

TAYLOR, RE ;

FULLMER, HM .

CLINICA CHIMICA ACTA, 1972, 42 (01) :43-+

[7]

SAKAMOTO S, 1972, P SOC EXP BIOL MED, V139, P1057

[8]

SEIFTER S, 1970, METHOD ENZYMOL, V19, P613

[9]

RELEASE OF COLLAGENASE AS AN INACTIVE PROENZYME BY BONE EXPLANTS IN CULTURE [J].

VAES, G .

BIOCHEMICAL JOURNAL, 1972, 126 (02) :275-&

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