APPLICATION OF A SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF MELPHALAN IN THE PRESENCE OF ITS HYDROLYSIS PRODUCTS

被引：43

作者：

FLORA, KP ^{[1
]}

SMITH, SL ^{[1
]}

CRADOCK, JC ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,COLL PHARM,PULLMAN,WA 99163

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 177卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)92602-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A procedure for the separation and quantitation of melphalan (l-PAM) and its hydrolysis products by high-performance liquid chromatography is described. The hydrolysis of l-PAM at 25 ± 0.1° and 41 ± ° was studied between pH 3.0 and 9.0. The pattern of hydrolysis suggested that l-PAM decomposes via two consecutive pseudo first-order reactions. Pseudo first-order rate constants (k1) were determined for the dissapearance of l-PAM at various pH values in buffered solutions and in a formulated product. At both temperature l-PAM solutions were found to be most stable at low pH. Chloride ionw as found to reduce the rate of hydrolysis. © 1979.

引用

页码：91 / 97

页数：7

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