2 CIS-ACTING REGULATORY SEQUENCES IN THE PEROXISOME PROLIFERATOR-RESPONSIVE ENHANCER REGION OF RAT ACYL-COA OXIDASE GENE

被引：202

作者：

OSUMI, T ^{[1
]}

WEN, JK ^{[1
]}

HASHIMOTO, T ^{[1
]}

机构：

[1] SHINSHU UNIV,SCH MED,DEPT BIOCHEM,MATSUMOTO,NAGANO 390,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91645-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To clarify the mechanism of transcriptional induction of the rat liver acyl-CoA oxidase gene by hypolipidemic agents, we searched for cis-acting regulatory sequences in the 5′ upstream region of the gene by transfection studies. We found that the sequence between -639 and -472 acts as a peroxisome proliferator-responsive, tissue-specific enhancer. Footprint analysis revealed two protein binding sites in this region. One of these sequences exhibited a positive, whereas the other a negative, regulatory activity in transcription assays. © 1991.

引用

页码：866 / 871

页数：6

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