PRODUCTION OF A TRANSFORMING GROWTH FACTOR-BETA-LIKE GROWTH-FACTOR BY RSV-TRANSFORMED RAT CEREBRAL MICROVASCULAR ENDOTHELIAL-CELLS

被引：8

作者：

MOORADIAN, DL ^{[1
]}

DIGLIO, CA ^{[1
]}

机构：

[1] WAYNE STATE UNIV,SCH MED,DEPT PATHOL,DETROIT,MI 48201

来源：

TUMOR BIOLOGY | 1991年 / 12卷 / 03期

关键词：

TRANSFORMING GROWTH FACTOR-BETA; ENDOTHELIAL CELL; ROUS SARCOMA VIRUS;

D O I：

10.1159/000217702

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This study describes the preliminary characterization of a Rous sarcoma virus (RSV)-transformed rat cerebral microvascular endothelial cell (RCE-T1)-derived growth factor with biological properties similar to transforming growth factor-beta (TGF-beta). A significant portion of this RCE-T1-derived TGF-beta-like growth factor was secreted in an active form and its secretion was elevated 2- to 3-fold in late passage cultures comprised of rapidly growing, less differentiated cells exhibiting reduced sensitivity to the growth-inhibitory effects of exogenous TGF-beta-1. These data suggest that the RCE-T1 cell line may be a useful system in which to study the role of autocrine TGF-beta-production in normal and aberrant endothelial cell growth.

引用

页码：171 / 183

页数：13

共 44 条

[1] AN ACTIVATED FORM OF TRANSFORMING GROWTH FACTOR-BETA IS PRODUCED BY COCULTURES OF ENDOTHELIAL-CELLS AND PERICYTES [J].

ANTONELLIORLIDGE, A ;

SAUNDERS, KB ;

SMITH, SR ;

DAMORE, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (12) :4544-4548

[2] SARCOMA GROWTH-FACTOR FROM CONDITIONED MEDIUM OF VIRALLY TRANSFORMED-CELLS IS COMPOSED OF BOTH TYPE-ALPHA AND TYPE-BETA TRANSFORMING GROWTH-FACTORS [J].

ANZANO, MA ;

ROBERTS, AB ;

SMITH, JM ;

SPORN, MB ;

DELARCO, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (20) :6264-6268

[3] INCREASED SECRETION OF TYPE-BETA TRANSFORMING GROWTH-FACTOR ACCOMPANIES VIRAL TRANSFORMATION OF CELLS [J].

ANZANO, MA ;

ROBERTS, AB ;

DELARCO, JE ;

WAKEFIELD, LM ;

ASSOIAN, RK ;

ROCHE, NS ;

SMITH, JM ;

LAZARUS, JE ;

SPORN, MB .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (01) :242-247

[4] TYPE-BETA TRANSFORMING GROWTH-FACTOR IN HUMAN-PLATELETS - RELEASE DURING PLATELET DEGRANULATION AND ACTION ON VASCULAR SMOOTH-MUSCLE CELLS [J].

ASSOIAN, RK ;

SPORN, MB .

JOURNAL OF CELL BIOLOGY, 1986, 102 (04) :1217-1223

[5] INHIBITION OF ENDOTHELIAL-CELL PROLIFERATION BY TYPE BETA-TRANSFORMING GROWTH-FACTOR - INTERACTIONS WITH ACIDIC AND BASIC FIBROBLAST GROWTH-FACTORS [J].

BAIRD, A ;

DURKIN, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 138 (01) :476-482

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7]

DELARCO JE, 1978, P NATL ACAD SCI USA, V75, P4001

[8] HUMAN TRANSFORMING GROWTH FACTOR-BETA COMPLEMENTARY-DNA SEQUENCE AND EXPRESSION IN NORMAL AND TRANSFORMED-CELLS [J].

DERYNCK, R ;

JARRETT, JA ;

CHEN, EY ;

EATON, DH ;

BELL, JR ;

ASSOIAN, RK ;

ROBERTS, AB ;

SPORN, MB ;

GOEDDEL, DV .

NATURE, 1985, 316 (6030) :701-705

[9] CULTURED ENDOTHELIAL-CELLS PRODUCE MULTIPLE GROWTH-FACTORS FOR CONNECTIVE-TISSUE CELLS [J].

DICORLETO, PE .

EXPERIMENTAL CELL RESEARCH, 1984, 153 (01) :167-172

[10] RAT HEART-DERIVED ENDOTHELIAL AND SMOOTH-MUSCLE CELL-CULTURES - ISOLATION, CLONING AND CHARACTERIZATION [J].

DIGLIO, CA ;

GRAMMAS, P ;

GIACOMELLI, F ;

WIENER, J .

TISSUE & CELL, 1988, 20 (04) :477-492

← 1 2 3 4 5 →