CONCLUSIVE EVIDENCE THAT VERY LONG-CHAIN FATTY-ACIDS ARE OXIDIZED EXCLUSIVELY IN PEROXISOMES IN HUMAN SKIN FIBROBLASTS

被引:33
作者
JAKOBS, BS
WANDERS, RJA
机构
[1] UNIV HOSP AMSTERDAM,DEPT CLIN BIOCHEM,MEIBERGDREEF 9,1105 AZ AMSTERDAM,NETHERLANDS
[2] UNIV HOSP AMSTERDAM,DEPT PEDIAT,1105 AZ AMSTERDAM,NETHERLANDS
关键词
D O I
10.1016/0006-291X(91)90967-C
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated the contribution of peroxisomes and mitochondria to the β-oxidation of palmitate (C16:0) and cerotate (C26:0) in intact human skin fibroblasts. The oxidation of both fatty acids was found to be inhibited by rotenone plus antimycin and cyanide, respectively, although to a different extent. When 2-[5-(4-chlorophenyl)pentyl]-oxirane-2-carboxylate (POCA) was used to specifically block carnitine palmitoyltransferase I, it was found that palmitate β-oxidation was inhibited almost completely whereas cerotate β-oxidation was not affected. Since carnitine palmitoyltransferase is essential for the oxidation of fatty acids in mitochondria this result provides conclusive evidence that oxidation of very-long-chain fatty acids is initiated in peroxisomes and not in mitochondria. © 1991.
引用
收藏
页码:842 / 847
页数:6
相关论文
共 17 条
[1]  
BREMER J, 1981, METHOD ENZYMOL, P506
[2]  
Lazarow P B, 1981, Methods Enzymol, V72, P315
[3]   FATTY ACYL-COA OXIDIZING SYSTEM IN RAT-LIVER PEROXISOMES - ENHANCEMENT BY CLOFIBRATE, A HYPOLIPIDEMIC DRUG [J].
LAZAROW, PB ;
DEDUVE, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (06) :2043-2046
[4]  
LAZAROW PB, 1989, METABOLIC BASIS INHE, P1501
[5]  
MANNAERTS GP, 1979, J BIOL CHEM, V254, P4585
[6]   TOTAL AND PEROXISOMAL OXIDATION OF VARIOUS SATURATED AND UNSATURATED FATTY-ACIDS IN RAT-LIVER, HEART AND MUSCULUS-QUADRICEPS [J].
REUBSAET, FAG ;
VEERKAMP, JH ;
TRIJBELS, JMF ;
MONNENS, LAH .
LIPIDS, 1989, 24 (11) :945-950
[7]  
REUBSAET FAG, 1991, IN PRESS BIOCH BIOPH
[8]   BETA-OXIDATION OF FATTY-ACIDS [J].
SCHULZ, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1081 (02) :109-120
[9]   MITOCHONDRIAL AND PEROXISOMAL BETA-OXIDATION OF STEARIC AND LIGNOCERIC ACIDS BY RAT-BRAIN [J].
SINGH, H ;
USHER, S ;
POULOS, A .
JOURNAL OF NEUROCHEMISTRY, 1989, 53 (06) :1711-1718
[10]   VERY LONG-CHAIN FATTY-ACID BETA-OXIDATION BY RAT-LIVER MITOCHONDRIA AND PEROXISOMES [J].
SINGH, H ;
DERWAS, N ;
POULOS, A .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 259 (02) :382-390