RNA SUBSTRATE BINDING-SITE IN THE CATALYTIC CORE OF THE TETRAHYMENA RIBOZYME

被引：205

作者：

PYLE, AM

MURPHY, FL

CECH, TR

机构：

[1] UNIV COLORADO, HOWARD HUGHES MED INST, BOULDER, CO 80309 USA

[2] UNIV COLORADO, DEPT CHEM & BIOCHEM, BOULDER, CO 80309 USA

来源：

NATURE | 1992年 / 358卷 / 6382期

关键词：

D O I：

10.1038/358123a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In catalysis by group I introns, the helix (P1) containing the RNA cleavage site must be positioned next to the guanosine binding site. We have identified a conserved adenine in the catalytic core that contributes to the stability of this arrangement and propose that it accepts a hydrogen bond from a specific 2'-OH in P1. Such base-backbone tertiary interactions may be generally important to the organization of RNA tertiary structure.

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页码：123 / 131

页数：9

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