THE INTERNALIZATION OF THE IGG2A ANTIGEN RECEPTOR DOES NOT REQUIRE THE ASSOCIATION WITH IG-ALPHA AND IG-BETA BUT THE ACTIVATION OF PROTEIN-TYROSINE KINASES DOES

被引:31
作者
WEISER, P [1 ]
RIESTERER, C [1 ]
RETH, M [1 ]
机构
[1] MAX PLANCK INST IMMUNBIOL,D-79108 FREIBURG,GERMANY
关键词
B CELL ANTIGEN RECEPTOR; SIGNAL TRANSDUCTION; ENDOCYTOSIS;
D O I
10.1002/eji.1830240327
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The B cell antigen receptor of class IgM is a multimeric protein complex containing the membrane-bound immunoglobulin molecule and a heterodimer of the two B cell-specific transmembrane proteins Ig-alpha and Ig-beta. The B cell antigen receptor fulfills a dual role on the surface of B cells. First, it is a signal transduction complex which can activate protein tyrosine kinases and induce the release of Ca2+ ions from intracellular stores. Second, its internalization mediates the specific uptake of bound antigens, which are processed intracellularly and presented as major histocompatibility complex-bound peptides on the cell surface. In case of the IgM antigen receptor, the association with the heterodimer is necessary for expression of large amounts of IgM on the surface. We show here that the IgG2a antigen receptor can be expressed on the surface of myeloma cells in two structurally different forms: either with or without the Ig-alpha/Ig-beta heterodimer. A functional comparison of the two forms of antigen receptors demonstrates that the Ig-alpha and Ig-beta molecules are required for the activation of protein tyrosine kinases after cross-linking of the B cell antigen receptor. In contrast, both forms of IgG2a are equally well internalized. This suggests that Ig-alpha and Ig-beta are essential for signal transduction through the IgG2a antigen receptor, whereas internalization can occur independently of the heterodimer.
引用
收藏
页码:665 / 671
页数:7
相关论文
共 55 条
[1]   TYROSINE-CONTAINING MOTIF THAT TRANSDUCES CELL ACTIVATION SIGNALS ALSO DETERMINES INTERNALIZATION AND ANTIGEN PRESENTATION VIA TYPE-III RECEPTORS FOR IGG [J].
AMIGORENA, S ;
SALAMERO, J ;
DAVOUST, J ;
FRIDMAN, WH ;
BONNEROT, C .
NATURE, 1992, 358 (6384) :337-341
[2]   PLASMA-MEMBRANE AND INTERNALIZED IMMUNOGLOBULINS OF LYMPH-NODE CELLS STUDIED WITH CONJUGATES OF ANTIBODY OR ITS FAB FRAGMENTS WITH HORSERADISH-PEROXIDASE [J].
ANTOINE, JC ;
AVRAMEAS, S ;
GONATAS, NK ;
STIEBER, A ;
GONATAS, JO .
JOURNAL OF CELL BIOLOGY, 1974, 63 (01) :12-23
[3]  
BOURGUIGNON LAW, 1985, INT REV CYTOL, V87, P195
[4]   2 DISTINCT MECHANISMS FOR REDISTRIBUTION OF LYMPHOCYTE SURFACE MACROMOLECULES .1. RELATIONSHIP TO CYTOPLASMIC MYOSIN [J].
BRAUN, J ;
FUJIWARA, K ;
POLLARD, TD ;
UNANUE, ER .
JOURNAL OF CELL BIOLOGY, 1978, 79 (02) :409-418
[5]   PROTEIN TYROSINE PHOSPHORYLATION IN INDUCED IN MURINE LYMPHOCYTES-B IN RESPONSE TO STIMULATION WITH ANTIIMMUNOGLOBULIN [J].
CAMPBELL, MA ;
SEFTON, BM .
EMBO JOURNAL, 1990, 9 (07) :2125-2131
[6]  
CHERAYIL BJ, 1993, J IMMUNOL, V151, P11
[7]  
CLARK MR, 1992, J IMMUNOL, V149, P2857
[8]   RELEASE OF A MEMBRANE-SURFACE GLYCOPROTEIN FROM HUMAN PLATELETS BY PHOSPHATIDYLINOSITOL SPECIFIC PHOSPHOLIPASE(S)-C [J].
DHAR, A ;
SHUKLA, SD .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1091 (01) :15-21
[9]   ROLE OF THE TRANSMEMBRANE AND CYTOPLASMIC DOMAINS OF SURFACE IGM IN ENDOCYTOSIS AND SIGNAL TRANSDUCTION [J].
DUBOIS, PM ;
STEPINSKI, J ;
URBAIN, J ;
SIBLEY, CH .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1992, 22 (03) :851-857
[10]   DUAL ROLE OF THE TYROSINE ACTIVATION MOTIF OF THE IG-ALPHA PROTEIN DURING SIGNAL-TRANSDUCTION VIA THE B-CELL ANTIGEN RECEPTOR [J].
FLASWINKEL, H ;
RETH, M .
EMBO JOURNAL, 1994, 13 (01) :83-89