A BISULFITE METHOD OF 5-METHYLCYTOSINE MAPPING THAT MINIMIZES TEMPLATE DEGRADATION

被引：161

作者：

RAIZIS, AM ^{[1
]}

SCHMITT, F ^{[1
]}

JOST, JP ^{[1
]}

机构：

[1] FRIEDRICH MIESCHER INST,CH-4002 BASEL,SWITZERLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1204

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The bisulfite method is a highly sensitive approach to 5-methylcytosine mapping that utilizes the capability of the polymerase chain reaction to exponentially amplify DNA. We have observed that the bisulfite reaction results in a significant level of template degradation due to DNA depurination. Furthermore, our data suggest that the DNA fragmentation which occurs limits the sensitivity of the method. We describe a simple solution to limit degradation of the DNA template. (C) 1995 Academic Press, Inc.

引用

页码：161 / 166

页数：6

共 14 条

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