PREPARATIVE ISOLATION OF ISOENZYMES OF ADENOSINE DEAMINASE FROM BOVINE MUCOSA BY ION-EXCHANGE CHROMATOGRAPHY

被引：43

作者：

MURPHY, PM

NOONAN, M

COLLINS, P

TULLY, E

BRADY, TG

机构：

[1] Department of Biochemistry, University College, Cork

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 171卷 / 01期

关键词：

D O I：

10.1016/0005-2744(69)90115-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1.A modified method, suitable for purifying larger quantities of adenosine deaminase (EC 3.5.4.4) is described. 2. 2.Separation by high-voltage electrophoresis provides a rapid analytical method for detecting the isoenzymes. 3. 3.The preparation in pure form of five isoenzymes by chromatography on Whatman DE 52 is described. 4. 4.Deamination rate, Km with adenosine and 6-chloropurine riboside, isoelectric point, denaturation by urea, heat stability, and inactivation by p-mercuribenzoate of the isoenzymes are compared. © 1969.

引用

页码：157 / &

共 13 条

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