IMPROVED DETECTION OF VIRUS-SPECIFIC IGM ANTIBODIES - ELIMINATION OF NONSPECIFIC IGM BINDING

被引:16
作者
SCHMITZ, H
机构
关键词
D O I
10.1099/0022-1317-40-2-459
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A non-specific reaction between human IgM and cytoplasmic structures of virus infected cells can often be observed if IgM antibodies to virus antigens are detected by indirect immunofluorescence or by immuno enzyme assays. Formaldehyde selectively inactivates the cytoplasmic receptors for human IgM without affecting the virus structural proteins. Alternatively, receptor-free antigens can be obtained by isolation of nuclei from virus infected cells. Due to reduced background, a more specific and more sensitive detection of IgM antibodies to Epstein-Barr virus, cytomegalovirus or central European encephalitis virus is possible.
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页码:459 / 463
页数:5
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