POSSIBLE MECHANISM RESPONSIBLE FOR THE CORRECTION OF TRANSCRIPTION ERRORS

被引:18
作者
VOLLOCH, VZ [1 ]
RITS, S [1 ]
TUMERMAN, L [1 ]
机构
[1] WEIZMANN INST SCI,REHOVOT 76100,ISRAEL
关键词
D O I
10.1093/nar/6.4.1535
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nucleoside triphosphate phosphohydrolase (NTPase) activity was found in a preparation of E. Coli RNA polymerase. This enzymatic activity is capaable of hydrolysing all four ribonucleoside triphosphates to the nucleoside diphosphates. However, during in vitro RNA synthesis directed by poly(dC) or poly(dT), only the non-complementary nucleoside triphosphate of the same heterocyclic class was hydrolysed. No incorporation of the non-complementary precursor into RNA could be detected in these experiments. When another RNA polymerase preparation, devoid of NTPase activity, was employed, there was no hydrolysis of any nucleoside triphosphate and significant incorporation of non-complemtary precursor into RNA was observed.These observations lead us to the conclusion that NTPase, acting in conjunction with RNA polymerase, has the function of correcting errors in transcription. © 1979 Information Retrieval Limited.
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页码:1535 / 1546
页数:12
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