QUANTITATIVE STUDY OF THE BINDING OF CYSTEINE PROTEINASES TO BASEMENT-MEMBRANES

被引：12

作者：

GUINEC, N

DALETFUMERON, V

PAGANO, M

机构：

[1] Laboratoire de Biochemie, Facultè de Mèdicine Broussais Hotel-Dieu, Universitè Pierre et Marie Curie, 75270 Paris Cedex 06

来源：

FEBS LETTERS | 1992年 / 308卷 / 03期

关键词：

CYSTEINE PROTEINASE; QUANTITATIVE BINDING; BASEMENT MEMBRANE;

D O I：

10.1016/0014-5793(92)81299-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for K(d) determination by competition experiments. The binding was characterized using the following parameters, the equilibrium constant, K(d), and the number of substrate sites, n, values of which were in the range of 10(-7) M and 10(12), respectively. Such results would be of significant interest for the understanding of the biological role of cysteine proteinases in tumour invasion and other types of tissue remodeling.

引用

页码：305 / 308

页数：4

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