Isolation of Plant DNA: A Fast, Inexpensive, and Reliable Method

被引：182

作者：

Guillemaut, Pierre ^{[1
]}

Marechal-Drouard, Laurence ^{[2
]}

机构：

[1] Inst Univ Technol Louis Pasteur, Lab Biol Vegetale Appl, F-67000 Strasbourg, France

[2] CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1992年 / 10卷 / 01期

关键词：

DNA isolation; woody plant; seed; cell culture;

D O I：

10.1007/BF02669265

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here a simple method to isolate DNA of high molecular weight from a wide variety of plant materials, such as trees, herbaceous plants, cell suspension cultures, calli, seeds, dried embryos, ferns and lichens. The crucial step of the extraction is the use of an acidic extraction medium. When necessary, the sample was separated on a fast RPC-5 column providing us with highly purified DNA suitable not only for restriction endonuclease analyses but also for PCR experiments, RLFP analyses, or detection of adducts.

引用

页码：60 / 65

页数：6

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