DETERMINATION OF PROANTHOCYANIDINS AND CATECHINS IN BEER AND BARLEY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH DUAL-ELECTRODE ELECTROCHEMICAL DETECTION

被引：84

作者：

MADIGAN, D

MCMURROUGH, I

SMYTH, MR

机构：

[1] GUINNESS BREWING WORLDWIDE RES CTR,DUBLIN 8,IRELAND

[2] DUBLIN CITY UNIV,SCH CHEM SCI,DUBLIN 9,IRELAND

来源：

ANALYST | 1994年 / 119卷 / 05期

关键词：

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; PROANTHOCYANIDINS AND CATECHINS; ELECTROCHEMICAL DETECTION; BEER; BARLEY;

D O I：

10.1039/an9941900863

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method was developed to determine the flavanols prodelphinidin B3, procyanidin B3, (+)-catechin, and (-)-epicatechin by high-performance liquid chromatography, using dual-channel electrochemical detection. This method was especially suited to the direct analysis of beer samples and to analysis of acetone extracts of barley samples, and was capable of determining proanthocyanidins and catechins at levels of 0.1-5.0 mg 1(-1). The use of dual-channel electrochemical detection also enabled unambiguous peak identification by measurement of collection efficiencies. This method offered improved sensitivity and selectivity compared with ultraviolet detection, and sample preparation procedures were greatly simplified. The method was applied to the comparison of stabilized and unstabilized lagers, and to the analysis of different barley varieties grown in Ireland.

引用

页码：863 / 868

页数：6

共 21 条

[1]

Gardner R. J., 1977, Technical Quarterly, Master Brewers Association of America, V14, P250

[2]

GRAMSHAW JW, 1973, J I BREWING, V79, P258

[3] COMPARISON OF ELECTROCHEMICAL AND ULTRAVIOLET DETECTION METHODS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE DETERMINATION OF PHENOLIC-COMPOUNDS COMMONLY FOUND IN BEERS .1. OPTIMIZATION OF OPERATING PARAMETERS [J].