CLONING AND CHARACTERIZATION OF A TRYPTOPHANASE GENE FROM ENTEROBACTER-AEROGENES SM-18

被引：14

作者：

KAWASAKI, K

YOKOTA, A

OITA, S

KOBAYASHI, C

YOSHIKAWA, S

KAWAMOTO, SI

TAKAO, S

TOMITA, F

机构：

[1] HOKKAIDO UNIV,FAC AGR,APPL MICROBIOL LAB,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

[2] HOKKAIDO UNIV,FAC AGR,MOLEC BIOL LAB,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-12-3275

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A tryptophanase gene from Enterobacter aerogenes SM-18 was cloned and sequenced. The structural gene for tryptophanase, tnaA, consisted of 1389 bp encoding 462 amino acid residues, and its nucleotide sequence and deduced amino acid sequence showed significant homology to those of tnaA from Escherichia coli K12. A short open reading frame consisting of 31 amino acid residues was found upstream of tnaA, and it showed some similarity to the E. coli tnaC gene known to be a cis-acting regulatory element for transcription. A partial open reading frame homologous to the 5' end of E. coli tnaB was observed at the 3'-flanking region of tnaA. These genes may thus constitute an operon as in E. coli.

引用

页码：3275 / 3281

页数：7

共 35 条

[1] INTRODUCTION OF BACTERIOPHAGE MU INTO PSEUDOMONAS-SOLANACEARUM AND RHIZOBIUM-MELILOTI USING R FACTOR RP4 [J].