Biosynthesis of silver nano-particles by marine sediment fungi for a dose dependent cytotoxicity against HEp2 cell lines

被引：32

作者：

Anand, Bibin G. ^{[1
]}

Thomas, C. K. Navin ^{[1
]}

Prakash, S. ^{[1
,2
]}

Kumar, C. Sathish ^{[3
]}

机构：

[1] Udaya Sch Engn, Dept Biotechnol, Vellamodi, Tamil Nadu, India

[2] Coll Marine Sci & Technol, Dept Marine Biotechnol, Massawa, North East Afri, Eritrea

[3] ILIFE BIOTECH Private Ltd, Madras, Tamil Nadu, India

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2015年 / 4卷 / 02期

关键词：

Marine sediment fungi; Silver nano-particles; Extracellar synthesis; HEp2 cell lines;

D O I：

10.1016/j.bcab.2015.01.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Biosynthesis of silver nanoparticles has been carried out by using the cell free filtrate of marine sediment fungal species of Southern peninsular coastal region of India and identified based on the sequence analysis of Internal Transcribed Spacer region of [-RNA genes. Species were identified as Aspergillus flavus SP-3, Trichoderrna garnsii SP-4, Talaromyces flavus SP-5, and Aspergillus oryzae SP-6. The phylogenetic relationship between the fungal isolates and other fungal strains was compared. Treatment of silver nitrate with the fungal extract produced stable, predominant, monoclispersed and spherical silver nanoparticles. Preliminary characterization revealed that the nano-particles were in the range of 20-60 am in all the fungal isolates. Among all the fungi the silver nano-particles of T. garnsa SP-4 showed an enhanced antimicrobial activity against Gram positive bacteria, Gram negative bacteria and fungi pathogens; an anti-oxidant activity at an effective concentration of 99 [igiml; and a close dependent cytotoxic activity against HEp2 cell lines at LC50 value of 23 [iginal. Thus, the strains, 5E33, 5P4, 5P5, and 5P6 could be used for simple, non-hazardous and efficient synthesis of antimicrobial and HEp2 cytotoxic silver nano-particles. (C) 2015 Elsevier Ltd. All rights reserved

引用

页码：150 / 157

页数：8

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