DNA METHYLATION AND DEMETHYLATION EVENTS DURING MEIOTIC PROPHASE IN THE MOUSE TESTIS

被引:72
作者
TRASLER, JM
HAKE, LE
JOHNSON, PA
ALCIVAR, AA
MILLETTE, CF
HECHT, NB
机构
[1] TUFTS UNIV,DEPT BIOL,MEDFORD,MA 02155
[2] HARVARD UNIV,SCH MED,HUMAN REPROD & REPROD BIOL LAB,BOSTON,MA 02115
关键词
D O I
10.1128/MCB.10.4.1828
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The genes encoding three different mammalian testis-specific nuclear chromatin proteins, mouse transition protein 1, mouse protamine 1, and mouse protamine 2, all of which are expressed postmeiotically, are marked by methylation early during spermatogenesis in the mouse. Analysis of DNA from the testes of prepubertal mice and isolated testicular cells revealed that transition protein 1 became progressively less methylated during spermatogenesis, while the two protamines became progressively more methylated; in contrast, the methylation of β-actin, a gene expressed throughout spermatogenesis, did not change. These findings provide evidence that both de novo methylation and demethylation events are occurring after the completion of DNA replication, during meiotic prophase in the mouse testis.
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页码:1828 / 1834
页数:7
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