CAPSULATION GENE LOSS AND RESCUE MUTATIONS DURING THE CAP+ TO CAP- TRANSITION IN HAEMOPHILUS-INFLUENZAE TYPE-B

被引：9

作者：

BROPHY, LN ^{[1
]}

KROLL, JS ^{[1
]}

FERGUSON, DJP ^{[1
]}

MOXON, ER ^{[1
]}

机构：

[1] JOHN RADCLIFFE HOSP,NUFFIELD DEPT PATHOL,OXFORD OX3 9DU,ENGLAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1991年 / 137卷

关键词：

D O I：

10.1099/00221287-137-11-2571

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genes for Haemophilus influenzae type b capsule expression are duplicated to form a potentially unstable structure, cap, of directly-repeated chromosomal regions of approximately 17 kb. Capsule-deficient mutants arise in a two-stage process, initiated by rec-dependent reduction of this region from two copies to one. This recombinational event is usually lethal, only about 1/200 surviving to form slow-growing colonies of organisms that continue to synthesize polysaccharide but are defective in its export. A variety of secondary 'rescue' mutations within cap can occur to reduce polysaccharide synthesis and restore normal organism appearance and colony morphology.

引用

页码：2571 / 2576

页数：6

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