QUANTIFICATION OF MONOCLONAL-ANTIBODIES IN COMPLEX-MIXTURES BY PROTEIN-G HIGH-PERFORMANCE LIQUID AFFINITY-CHROMATOGRAPHY

被引：10

作者：

BLANK, GS

VETTERLEIN, D

机构：

[1] Department of Recovery Process R and D, Genentech, Inc., South San Francisco, CA 94080

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 190卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90201-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance liquid affinity chromatography (HPLAC) utilizing Protein G as a ligand has been evaluated for rapid quantification of monoclonal antibodies (MAbs) in various solutions. The results obtained by HPLAC agreed to within 10% of a standard enzyme-linked immunospecific assay (ELISA). A standard curve was prepared by injection of known amounts of a purified murine IgG1 with the elution peak area analyzed by computer integration software. Accuracy of quantification was independent of the injection volume, solution composition, or mouse IgG subclass. A method is described for using Protein G HPLAC to determine murine IgG levels in various complex mixtures within 15 min, compared to the ELISA which required 5 h. © 1990.

引用

页码：317 / 320

页数：4

共 15 条

[1]

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[2]

AKERSTROM B, 1986, J BIOL CHEM, V261, P240

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