PHYLOGENY OF LYMPHOCYTE HETEROGENEITY .3. MITOGENIC RESPONSES OF REPTILIAN LYMPHOCYTES

被引：27

作者：

CUCHENS, MA

CLEM, LW

机构：

[1] UNIV FLORIDA, COLL MED, DEPT IMMUNOL & MED MICROBIOL, GAINESVILLE, FL 32611 USA

[2] UNIV FLORIDA, WHITNEY MARINE LAB, GAINESVILLE, FL 32611 USA

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 1979年 / 3卷 / 02期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0145-305X(79)80025-7

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Peripheral blood lymphocytes from Florida alligators were isolated and observed to undergo mitogenesis when cultured in vitro with Con A, PHA, LPS, PWM and PPD. These cells were also observed to participate in two-way mixed lymphocyte reactions. Optimal culture conditions included using mammalian medium (MEM) supplemented with adult alligator serum and additional sodium chloride and incubation at 32°C. In contrast to previous studies with lymphocytes from another ectotherm, the bluegill, a bony fish, alligator lymphocytes exhibited no differential effects of incubation temperatures when different mitogens were employed. © 1979 Pergamon Press Ltd. All rights reserved.

引用

页码：287 / 297

页数：11

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