PURIFICATION AND CHARACTERIZATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NEF GENE-PRODUCT EXPRESSED BY A RECOMBINANT BACULOVIRUS

被引：30

作者：

MATSUURA, Y

MAEKAWA, M

HATTORI, S

IKEGAMI, N

HAYASHI, A

YAMAZAKI, S

MORITA, C

TAKEBE, Y

机构：

[1] NATL INST HLTH,AIDS RES CTR,MUSASHIMURAYAMA,TOKYO 208,JAPAN

[2] OSAKA NATL HOSP,DEPT CLIN RES,OSAKA 540,JAPAN

[3] UNIV TOKYO,DEPT PURE & APPL SCI,MEGURO KU,TOKYO 153,JAPAN

来源：

VIROLOGY | 1991年 / 184卷 / 02期

关键词：

D O I：

10.1016/0042-6822(91)90428-E

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have constructed the recombinant baculovirus which expresses the human immunodeficiency virus type 1 negative factor (nef) gene. Spodoptera frugiperda cells infected with the recombinant virus produced a 27-kDa protein which reacted with rabbit antisera raised against a carboxy-terminal synthetic peptide of the Nef protein by immunoblot analysis. Labeling experiment showed that the recombinant Nef protein was myristoylated. The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 413, blue-Sepharose, and Sephadex G-150 column chromatography. No detectable GTP binding activity was observed in the purified recombinant Nef product. © 1991.

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页码：580 / 586

页数：7

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