LARGE AREA REAL-TIME SYSTEM FOR SCREENING DNA LIBRARIES

被引：4

作者：

GORDON, JS

MCGANN, W

DALEY, K

MCCONCHIE, L

SQUILLANTE, MR

机构：

[1] Radiation Monitoring Devices, Inc., Watertown, MA 02172

[2] Dept. of Physics, Univ. of Hawaii, Honolulu

来源：

IEEE TRANSACTIONS ON NUCLEAR SCIENCE | 1992年 / 39卷 / 05期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1109/23.173229

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A nuclear imaging system based on a position-sensitive photomultiplier tube and scintillating fiber optics is described. Its target application is the detection of radiolabeled DNA fragments as an aid in the isolation and harvesting of clones for sequencing the human genome. The system represents a substantial improvement in acquisition speed and dynamic range over the current method, autoradiography, while remaining competitive in terms of resolution. The digital nature of the image allows a great deal of freedom in processing and displaying data, including automatic identification and location of the desired features. Ultimately this system will be made compatible with a fully automated DNA processing system.

引用

页码：1485 / 1489

页数：5

共 5 条

[1]

BOUTOT JP, 1983, ADV ELECTRON EL PHYS, V60, P223

[2] NEWLY DEVELOPED PHOTOMULTIPLIER TUBES WITH POSITION SENSITIVITY CAPABILITY [J].