Biolistic transformation of cucumber using embryogenic suspension cultures: Long-term expression of reporter genes

被引:19
作者
Schulze, J
Balko, C
Zellner, B
Koprek, T
Hansch, R
Nerlich, A
Mendel, RR
机构
[1] TECH UNIV CAROLO WILHELMINA BRAUNSCHWEIG, INST BOT, D-38106 BRAUNSCHWEIG, GERMANY
[2] FED CTR BREEDING RES CULTIVATED PLANTS, INST STRESS PHYSIOL & QUAL RAW MAT, D-18190 GROSS LUSEWITZ, GERMANY
关键词
cucumber; transformation; particle gun; transgenic plants; nptII; uidA; kanamycin; long term gene expression;
D O I
10.1016/0168-9452(95)04261-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The generation of transgenic cucumber (Cucunis sativus L.) plants was achieved by biolistic transformation of a highly embryogenic cell suspension culture using the nptII and uidA gene. Functional expression of the genes in transgenic plants was determined by neomycin phosphotransferase and beta-glucuronidase enzyme assays. Southern analysis of DNA isolated from kanamycin-resistant plants confirmed stable integration of the genes as well as multicopy integration and rearrangements, A study of gene expression showed activity of the uidA gene in plants regenerated from kanamycin-resistant calli about one year after bombardment, indicating a high stability of the nonselectable gene.
引用
收藏
页码:197 / 206
页数:10
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