Biolistic transformation of cucumber using embryogenic suspension cultures: Long-term expression of reporter genes

被引：19

作者：

Schulze, J

Balko, C

Zellner, B

Koprek, T

Hansch, R

Nerlich, A

Mendel, RR

机构：

[1] TECH UNIV CAROLO WILHELMINA BRAUNSCHWEIG, INST BOT, D-38106 BRAUNSCHWEIG, GERMANY

[2] FED CTR BREEDING RES CULTIVATED PLANTS, INST STRESS PHYSIOL & QUAL RAW MAT, D-18190 GROSS LUSEWITZ, GERMANY

来源：

PLANT SCIENCE | 1995年 / 112卷 / 02期

关键词：

cucumber; transformation; particle gun; transgenic plants; nptII; uidA; kanamycin; long term gene expression;

D O I：

10.1016/0168-9452(95)04261-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The generation of transgenic cucumber (Cucunis sativus L.) plants was achieved by biolistic transformation of a highly embryogenic cell suspension culture using the nptII and uidA gene. Functional expression of the genes in transgenic plants was determined by neomycin phosphotransferase and beta-glucuronidase enzyme assays. Southern analysis of DNA isolated from kanamycin-resistant plants confirmed stable integration of the genes as well as multicopy integration and rearrangements, A study of gene expression showed activity of the uidA gene in plants regenerated from kanamycin-resistant calli about one year after bombardment, indicating a high stability of the nonselectable gene.

引用

页码：197 / 206

页数：10

共 42 条

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BALKO C, 1990, THESIS U HALLEWITTEN

[2] ORGANOGENESIS VERSUS EMBRYOGENESIS FROM LONG-TERM SUSPENSION-CULTURES OF CUCUMBER (CUCUMIS-SATIVUS L) [J].