UNCOUPLING OF RNA AND DNA-SYNTHESIS AFTER PLASMA STIMULATION OF G0-ARRESTED BALB-C-3T3 CELLS

被引：11

作者：

ABELSON, HT

ANTONIADES, HN

SCHER, CD

机构：

[1] HARVARD UNIV,CTR BLOOD RES,BOSTON,MA 02115

[2] HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115

[3] HARVARD UNIV,SCH MED,SIDNEY FARBER CANC INST,DEPT PEDIAT,BOSTON,MA 02115

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 561卷 / 02期

关键词：

(3T3 cell); Cell cycle; DNA synthesis; Growth factor; Plasma stimulation; RNA synthesis;

D O I：

10.1016/0005-2787(79)90136-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The addition of whole serum to G0-arrested, confluent Balb c-3T3 cells induces them to progress through G1 and synthesize DNA after a 12-h lag period. Prior to the onset of DNA synthesis, RNA is synthesized and RNA content increases. Serum has been fractionated into two sets of growth factors: a platelet-derived growth factor present in heat-treated (100°C) platelet extracts and platelet-poor plasma. Addition of whole serum, platelet-derived growth factor or platelet-poor plasma induces quiescent cells to increase their cytoplasmic RNA content, but the cells treated with platelet-poor plasma do not synthesize DNA. Messenger RNA content increases within 2 h after stimulation with whole serum or platelet-poor plasma, and after 18 h, mRNA has accumulated to a greater degree than rRNA. © 1979.

引用

页码：269 / 275

页数：7

共 12 条

[1] CHANGES IN RNA IN RELATION TO GROWTH OF FIBROBLAST .2. LIFETIME OF MESSENGER-RNA, RIBOSOMAL-RNA, AND TRANSFER-RNA IN RESTING AND GROWING CELLS [J].