A CHYMOTRYPSIN-LIKE PROTEASE INVOLVES IN MOTILITY OF SPERM IN SALMONID FISH

被引：23

作者：

INABA, K

MORISAWA, M

机构：

[1] Misaki Marine Biological Station, Faculty of Science, University of Tokyo, Miura

来源：

BIOMEDICAL RESEARCH-TOKYO | 1991年 / 12卷 / 06期

关键词：

D O I：

10.2220/biomedres.12.435

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Ten kinds of protease inhibitors were examined for their effects on motility of the demembranated sperm of chum salmon. Among them, aprotinin, N-tosyl-phenylalanyl-chloromethane (TPCK) and chymostatin showed strong inhibitory effects on the sperm motility. The inhibitory effects of these three protease inhibitors were observed only when the demembranated sperm were reactivated with relatively high concentration of ATP (> 0.5 mM). The results suggest that a chymotrypsin-like protease involves in sperm motility in an ATP-dependent manner.

引用

页码：435 / 437

页数：3

共 17 条

[1]

BACCETTI B, 1976, MONOGRAPHS DEV BIOL, P34

[2] TROUT SPERM MOTILITY - THE TRANSIENT MOVEMENT OF TROUT SPERM IS RELATED TO CHANGES IN THE CONCENTRATION OF ATP FOLLOWING THE ACTIVATION OF THE FLAGELLAR MOVEMENT [J].

CHRISTEN, R ;

GATTI, JL ;

BILLARD, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 166 (03) :667-671

[3] PROTEASE INHIBITOR AND SUBSTRATES BLOCK MOTILITY AND MICROTUBULE SLIDING OF SEA-URCHIN AND CARP SPERMATOZOA [J].

COSSON, MP ;

GAGNON, C .

CELL MOTILITY AND THE CYTOSKELETON, 1988, 10 (04) :518-527

[4] EFFECTS OF PROTEASE INHIBITORS AND SUBSTRATES ON MOTILITY OF MAMMALIAN SPERMATOZOA [J].

DELAMIRANDE, E ;

GAGNON, C .

JOURNAL OF CELL BIOLOGY, 1986, 102 (04) :1378-1383

[5] SKELETAL-MUSCLE PROTEASOME CAN DEGRADE PROTEINS IN AN ATP-DEPENDENT PROCESS THAT DOES NOT REQUIRE UBIQUITIN [J].

DRISCOLL, J ;

GOLDBERG, AL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (03) :787-791

[6] FLAGELLAR MOVEMENT AND ADENOSINE-TRIPHOSPHATASE ACTIVITY IN SEA-URCHIN SPERM EXTRACTED WITH TRITON X-100 [J].

GIBBONS, BH ;

GIBBONS, IR .

JOURNAL OF CELL BIOLOGY, 1972, 54 (01) :75-+

[7] EFFECTS OF PROTEASE INHIBITORS ON SPERM-RELATED EVENTS IN SEA-URCHIN FERTILIZATION [J].

GREEN, JD ;

SUMMERS, RG .

DEVELOPMENTAL BIOLOGY, 1982, 93 (01) :139-144

[8] EVIDENCE FOR PARTICIPATION OF SPERM PROTEINASES IN FERTILIZATION OF THE SOLITARY ASCIDIAN, HALOCYNTHIA-RORETZI - EFFECTS OF PROTEASE INHIBITORS [J].

HOSHI, M ;

NUMAKUNAI, T ;

SAWADA, H .

DEVELOPMENTAL BIOLOGY, 1981, 86 (01) :117-121

[9] PROTEASOME (MULTICATALYTIC PROTEINASE) OF SEA-URCHIN SPERM AND ITS POSSIBLE PARTICIPATION IN THE ACROSOME REACTION [J].

MATSUMURA, K ;

AKETA, K .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 1991, 29 (02) :189-199

[10] CYCLIC-AMP INDUCES MATURATION OF TROUT SPERM AXONEME TO INITIATE MOTILITY [J].

MORISAWA, M ;

OKUNO, M .

NATURE, 1982, 295 (5851) :703-704

← 1 2 →