EVALUATION OF A RAPID DOT-ELISA AS A FIELD-TEST FOR THE DIAGNOSIS OF CYSTIC HYDATID-DISEASE

被引：67

作者：

ROGAN, MT

CRAIG, PS

ZEYHLE, E

ROMIG, T

LUBANO, GM

DESHAN, L

机构：

[1] AMREF,WILSON AIRPORT,NAIROBI,KENYA

[2] KEMRI,NAIROBI,KENYA

[3] LANZHOU MED COLL,DEPT PARASITOL,LANZHOU,PEOPLES R CHINA

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1991年 / 85卷 / 06期

关键词：

D O I：

10.1016/0035-9203(91)90451-4

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A rapid dot enzyme-linked immunosorbent assay (dot-ELISA) was developed as a field test for the diagnosis of cystic hydatid disease caused by Echinococcus granulosus. The 30 min test was based on the detection of antibodies to antigen B of hydatid fluid and was carried out using 50-mu-l of whole blood in a field assessment in the Turkana region of north-west Kenya. Initial laboratory studies showed antigen B to be preferable to crude cyst fluid, with 94% sensitivity and 90.3% specificity for Echinococcus infections. The field test was rapid, inexpensive and simple to perform and is considered to be a useful back-up to ultrasound scanning.

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收藏

页码：773 / 777

页数：5

相关论文

共 34 条

[1] IMMUNODIAGNOSIS OF TAENIASIS BY COPROANTIGEN DETECTION [J].

ALLAN, JC ;

AVILA, G ;

NOVAL, JG ;

FLISSER, A ;

CRAIG, PS .

PARASITOLOGY, 1990, 101 :473-477

[2] STANDARDIZATION AND EVALUATION OF AN ENZYME-IMMUNOASSAY AS A SCREENING-TEST FOR THE SEROEPIDEMIOLOGY OF HUMAN HYDATIDOSIS [J].

COLTORTI, EA .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1986, 35 (05) :1000-1005

[3] HYDATID-DISEASE IN CHINA [J].

CRAIG, PS ;

DESHAN, L ;

ZHAOXUN, D .

PARASITOLOGY TODAY, 1991, 7 (02) :46-50

[4] HYDATID-DISEASE - RESEARCH AND CONTROL IN TURKANA .2. THE ROLE OF IMMUNOLOGICAL TECHNIQUES FOR THE DIAGNOSIS OF HYDATID-DISEASE [J].

CRAIG, PS ;

ZEYHLE, E ;

ROMIG, T .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1986, 80 (02) :183-192

[5] THE IDENTIFICATION OF EGGS OF ECHINOCOCCUS BY IMMUNOFLUORESCENCE USING A SPECIFIC ANTI-ONCOSPHERAL MONOCLONAL-ANTIBODY [J].

CRAIG, PS ;

MACPHERSON, CNL ;

NELSON, GS .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1986, 35 (01) :152-158

[6]

FELGNER P, 1978, TROPENMED PARASITOL, V29, P417

[7] NEW APPROACHES IN THE DIAGNOSIS OF TAENIA-SOLIUM CYSTICERCOSIS AND TAENIASIS [J].

FLISSER, A ;

PLANCARTE, A ;

CORREA, D ;

RODRIGUEZDELROSAL, E ;

FELDMAN, M ;

SANDOVAL, M ;

TORRES, A ;

MEZA, A ;

PARKHOUSE, RME ;

HARRISON, LJS ;

WILSON, M ;

AVILA, G ;

ALLAN, J ;

CRAIG, PS ;

VALLEJO, V ;

ORTIZ, D ;

GARCIA, E ;

MCMANUS, DP .

ANNALES DE PARASITOLOGIE HUMAINE ET COMPAREE, 1990, 65 :95-98

[8] HYDATID-DISEASE IN THE TURKANA DISTRICT OF KENYA .5. PROBLEMS OF INTERPRETATION OF DATA FROM A MASS SEROLOGICAL SURVEY [J].

FRENCH, CM ;

INGERA, WE .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1984, 78 (03) :213-218

[9] SEROLOGICAL DIFFERENTIATION BETWEEN ECHINOCOCCUS-GRANULOSUS AND ECHINOCOCCUS-MULTILOCULARIS INFECTIONS IN MAN [J].

GOTTSTEIN, B ;

ECKERT, J ;

FEY, H .

ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 1983, 69 (03) :347-356

[10]

GOTTSTEIN B, 1987, PARASITOL RES, V73, P196

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